USP16 antibody

Catalog No. ABIN7120500

This anti-USP16 antibody is a Rabbit Polyclonal antibody detecting USP16 in WB, ELISA, IHC and IP. Suitable for Human, Mouse and Rat.

Quick Overview for USP16 antibody (ABIN7120500)

Target: USP16 (Ubiquitin Specific Peptidase 16 (USP16))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP16 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-USP16 Antibody

Purpose: USP16 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ubiquitin specific peptidase 16

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IP: 1:500-1:5000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for USP16

Target: USP16 (Ubiquitin Specific Peptidase 16 (USP16))

Alternative Name: USP16

Background:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 16|Deubiquitinating enzyme 16|Ubiquitin thioesterase 16|Ubiquitin-processing protease UBP-M|Ubiquitin-specific-processing protease 16|USP16

Background: Specifically deubiquitinates 'Lys-120' of histone H2A(H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3(H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B-and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.

Molecular Weight: 94 kDa

Gene ID: 10600

UniProt: Q9Y5T5